With this launch, the company's 5G services are available in 82 cities.
Smartphone models supporting the Standalone (SA) network have received a software update to work seamlessly on Reliance Jio's network, according to a statement.
Pune has a large student population and is an IT hub, as well as a prominent automobile and manufacturing hub, the company said, noting that "Jio True 5G will be a true game changer for Punekars".
Earlier, Jio 5G was successfully beta-launched in six cities - Mumbai, Delhi, Kolkata, Chennai, Varanasi and Nathdwara.
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